Tissue optics, Oxygen, hyperspectral imaging, multimode, burn

F. Vasefi, D.L. Farkas
Biomedical Optical Imaging Corporation, California, United States

Keywords: Differentiation between superficial and deep-dermal burns using Multimode Hyperspectral Imaging

Non-invasive, non-contact differentiation between superficial and deep-dermal burns is still challenging with current clinical procedures. These two types of burns require different treatment approaches, including the decision for excision and skin grafting procedures. Our aim is to assess the ability of Multimode Hyperspectral Imaging (MM-HSI) in differentiating burn depth. We developed and used a multimode skin imaging system that combines polarization, fluorescence and hyperspectral imaging to accurately map the 3-D distribution of skin melanin, collagen and oxy-deoxy hemoglobin in skin. The method combines two depth sensitive techniques: polarization and hyperspectral imaging, to accurately determine the spatial distribution of melanin and hemoglobin oxygenation in skin, including in and around skin lesions. By quantifying melanin absorption in pigmented area, we can more accurately estimate fluorescence emission distribution mainly from skin collagen (excited at 420 nm). The system has been originally developed for early melanoma detection by assessment of lesion melanin depth and related vasculature conditions. Since superficial burn injury causes an inflammation and increased perfusion, whereas deep-dermal burns destroy the dermal microvasculature and consequently decrease the perfusion, MM-HSI can be extremely useful to provide the clinical information to the clinician, non-invasively.