Ruggedized, Portable Perfusion System for Tissue Preservation

G. Srinivas
TDA Research Inc, United States

Keywords: normothermic machine perfusion, oxygen carrying perfusate, perfusion machine

Transplantable tissue is stored using static cold storage (SCS), which slows the cell's metabolism, minimizing ischemic injury, by cooling it to 4oC. Instead of slowing the metabolism via cold temperatures, normothermic machine perfusion (NMP) minimizes ischemic injury by maintaining the tissue's metabolic function ex-vivo while providing nutrients and oxygen. NMP facillitates tissues to be stored for longer than with SCS allowing the tissue to heal before transplantation, improving outcomes. Most perfusion systems in the literature require constant supervision, are not portable, and cannot handle extreme temperatures. An automated, robust, and portable, perfusion system would be a huge improvement. Tissues need oxygen to maintain their metabolism. Typically, blood is used as the perfusate because it has a built-in oxygen carrier (the red blood cells). In austere environments blood is not the answer because of a limited blood supply. TDA is developing a perfusion machine which will be portable, lightweight, and ruggedized to withstand extreme temperature variations. Our partner, Southwest Research Institute, is developing a universal oxygen carrier as a replacement for red blood cells in the perfusate. We are also collaborating with the University of North Carolina Charlotte to test our perfusion machine and novel perfusate in animal tissues.